Experiment on the effect of Artemisia sieversiana extract on hair loss prevention and cell growth

Objectives: This study aimed to examine the safety, effects on proliferation of hair papilla cells, and anti-inflammatory antioxidant mechanisms Artemisia sieversiana Ehrh. ex Willd. (AS) extract.Methods: Safety tests through purity testing, acute toxicity tests, repeated were performed using AS extract (ASE) which had been dried for over two years. Cell culture conducted; VEGF (vascular endothelial growth factor), bFGF (basic fibroblast EGF (epidermal factor) protein expression analyses mechanistic evaluation; inhibitory ASE RNA testosterone, 5α-reductase, aromatase was assessed. The efficacy confirmed by measuring levels nitric oxide, inflammatory mediators (TNF-α PGE2), cytokines (IL-1β, IL-6, IL-8), chemokine MCP-1.Results: safety confirmed. mechanism cell in human follicle dermal cells involved promotion VEGF, bFGF, expression. decreased mRNA aromatase-1 a concentration-dependent manner. PGE2 TNF-α production also significantly manner, cytokine inhibited.Conclusions: is suggested promote papillary at cellular level, suppress various enzymes cycle death, inhibit loss anti-androgen, anti-inflammatory, effects.